Does the PCR reaction volume (negatively) influence the outc
来源:学生作业帮 编辑:搜狗做题网作业帮 分类:英语作业 时间:2024/06/26 05:42:36
Does the PCR reaction volume (negatively) influence the outcome?
![Does the PCR reaction volume (negatively) influence the outc](/uploads/image/z/18057981-21-1.jpg?t=Does+the+PCR+reaction+volume+%28negatively%29+influence+the+outc)
博凌科为-为你No,especially since the introduction of the small,thin walled,0.2 ml plastic vials fitting the 96 well metal blocks of the thermocyclers.A number of observations are worth mentioning:if using older model thermocyclers (without a heated lid),to run small volume PCR reactions,mineral oil is necessary to cover the reaction mixture.Use of oil increases the volume of liquid in the vial and thus can influence somewhat the outcome.Besides,older thermocyclers require the use of larger plastic vials,with thicker walls,that fit less well in the metal blocks and thus may increase the likelihood of variation in PCr outcome.small,thin-walled plastic vials designed for the 96 well metal blocks are ideal for running small-volume PCR reactions.Due to the heated lid of the thermocyclers,there are no mineral oil requirements.When tested,reactions yielded similar results,whether the reaction volume was 100,25 or 5 microliters.It is important to mention,that small volume PCRs may be very beneficial when using small amounts of DNA template.In general,at a constant amount of template DNA,the yield of PCR product per microliter reaction is higher when the reaction volume is 5 L compared to 100 L.This may allow visualisation of the PCR products,sometimes invisible when larger reaction volumes are used.
How does the Internet influence our life?
PCR体系怎么配PCR was performed in a reaction volume of 25 ll cont
The specifide folder does not contain the correct volume
英语翻译The statement linking technology negatively with free th
What influence does the American Revolutionary War have on A
The volume is dirty
Find the volume.
to turn the volume
The influence of internet
under the influence
How does the media influence pop culture?How does music infl
英语翻译The p values for the temporal trends of in-hospital outc